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michaelis-menten non-linear regression with least-squares fit in prism 6.0  (GraphPad Software Inc)


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    GraphPad Software Inc michaelis-menten non-linear regression with least-squares fit in prism 6.0
    Michaelis Menten Non Linear Regression With Least Squares Fit In Prism 6.0, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/non-linear+regression+michaelis+menten+or+kcat+model/michaelis+menten+equation+graphpad+prism+9/us12366576-946-28-30
    Average 90 stars, based on 1 article reviews
    michaelis-menten non-linear regression with least-squares fit in prism 6.0 - by Bioz Stars, 2026-09
    90/100 stars

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    Phosphoserine 1589 of Fhod3 inhibits PRMT7 activity. 5 μg of GST-HsPRMT7, 0.14 μM of [ 3 H]AdoMet and indicated Fhod3 (1581-1595) peptides incubated at 20 °C for 1 h in a reaction mixture of 50 mM K-HEPES, 1 mM DTT, and pH of 8.5 in a final volume of 30 μl. A , Fhod3 (1581-1595) WT, ( B ) Fhod3 (1581-1595) poS1589, and ( C ) Fhod3 (1581-1595) po S1595. Curves were fitted to a standard <t>Michaelis-Menten</t> equation using GraphPad PRISM, n = 3.
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    Phosphoserine 1589 of Fhod3 inhibits PRMT7 activity. 5 μg of GST-HsPRMT7, 0.14 μM of [ 3 H]AdoMet and indicated Fhod3 (1581-1595) peptides incubated at 20 °C for 1 h in a reaction mixture of 50 mM K-HEPES, 1 mM DTT, and pH of 8.5 in a final volume of 30 μl. A , Fhod3 (1581-1595) WT, ( B ) Fhod3 (1581-1595) poS1589, and ( C ) Fhod3 (1581-1595) po S1595. Curves were fitted to a standard <t>Michaelis-Menten</t> equation using GraphPad PRISM, n = 3.
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    Phosphoserine 1589 of Fhod3 inhibits PRMT7 activity. 5 μg of GST-HsPRMT7, 0.14 μM of [ 3 H]AdoMet and indicated Fhod3 (1581-1595) peptides incubated at 20 °C for 1 h in a reaction mixture of 50 mM K-HEPES, 1 mM DTT, and pH of 8.5 in a final volume of 30 μl. A , Fhod3 (1581-1595) WT, ( B ) Fhod3 (1581-1595) poS1589, and ( C ) Fhod3 (1581-1595) po S1595. Curves were fitted to a standard Michaelis-Menten equation using GraphPad PRISM, n = 3.

    Journal: The Journal of Biological Chemistry

    Article Title: Methylation and phosphorylation of formin homology domain proteins (Fhod1 and Fhod3) by protein arginine methyltransferase 7 (PRMT7) and Rho kinase (ROCK1)

    doi: 10.1016/j.jbc.2024.107857

    Figure Lengend Snippet: Phosphoserine 1589 of Fhod3 inhibits PRMT7 activity. 5 μg of GST-HsPRMT7, 0.14 μM of [ 3 H]AdoMet and indicated Fhod3 (1581-1595) peptides incubated at 20 °C for 1 h in a reaction mixture of 50 mM K-HEPES, 1 mM DTT, and pH of 8.5 in a final volume of 30 μl. A , Fhod3 (1581-1595) WT, ( B ) Fhod3 (1581-1595) poS1589, and ( C ) Fhod3 (1581-1595) po S1595. Curves were fitted to a standard Michaelis-Menten equation using GraphPad PRISM, n = 3.

    Article Snippet: Michaelis Menten non-linear regression was performed using GraphPad Prism version 5.01 for Windows, GraphPad Software, www.graphpad.com .

    Techniques: Activity Assay, Incubation

    Phosphoserine 32 of human histone H2B abolishes PRMT7 activity. 5 μg of GST-HsPRMT7 was mixed with either ( A ) H2B (23-37) WT, ( B ) H2B (23-37) po S32 or ( C ) H2B (23-37) po S36, and 0.7 μM [ 3 H]AdoMet. Reaction mixtures contained 50 mM K-HEPES, 1 mM DTT, pH = 8.5 in a final volume of 30 μl. Reactions were incubated at 20 °C for 1 h prior to termination with 100% TFA. Points were fitted to a standard Michaelis Menten fit equation using GraphPad PRISM, n = 3.

    Journal: The Journal of Biological Chemistry

    Article Title: Methylation and phosphorylation of formin homology domain proteins (Fhod1 and Fhod3) by protein arginine methyltransferase 7 (PRMT7) and Rho kinase (ROCK1)

    doi: 10.1016/j.jbc.2024.107857

    Figure Lengend Snippet: Phosphoserine 32 of human histone H2B abolishes PRMT7 activity. 5 μg of GST-HsPRMT7 was mixed with either ( A ) H2B (23-37) WT, ( B ) H2B (23-37) po S32 or ( C ) H2B (23-37) po S36, and 0.7 μM [ 3 H]AdoMet. Reaction mixtures contained 50 mM K-HEPES, 1 mM DTT, pH = 8.5 in a final volume of 30 μl. Reactions were incubated at 20 °C for 1 h prior to termination with 100% TFA. Points were fitted to a standard Michaelis Menten fit equation using GraphPad PRISM, n = 3.

    Article Snippet: Michaelis Menten non-linear regression was performed using GraphPad Prism version 5.01 for Windows, GraphPad Software, www.graphpad.com .

    Techniques: Activity Assay, Incubation